解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
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病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
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放射学,核医学和医学成像
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生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The appearance of RNP droplets associated with specific regions of polytene chromosomes from Chironomus larvae has been studied, systematically by light microscopy using glutarladehyde fixation and lactic acid squashing of the salivary gland. During the fourth larval instar the highest frequency of nuclei which contai...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00344485
更新日期:1979-09-01 00:00:00
abstract::Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00331572
更新日期:1979-08-10 00:00:00
abstract::Chromosomes were subjected to either prolonged hypotonic solution pretreatment or aging. Both conditions greatly loosened and dispersed the overlying epichromatin from the central chromosome core structure. This was followed by silver staining and examination with bright-field microscopy. The chromosome core selective...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294845
更新日期:1979-06-21 00:00:00
abstract::Metaphase chromosomes from the Chinese hamster cell line M3-1 were separated by means of a flow sorter. Two chromosome fractions were used for this study: A, which consisted of 95% pure chromosome no. 1, and B, which was 90% pure chromosome no. 2. The DNA of 10(6) chromosomes of each type was purified, and a 125I-cRNA...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328767
更新日期:1979-01-31 00:00:00
abstract::The 5S RNA gene content of polyploid cells of the ovary of Drosophila melanogaster has been compared in animals with two or three gene clusters. The amount of 5S RNA genes is exactly proportional to the number of gene clusters as determined by DNA-RNA filter hybridization. In contrast, the number of rDNA genes in endo...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328768
更新日期:1979-01-31 00:00:00
abstract::Rates of movement of univalents at prometaphase and of half-bivalents at anaphase in living cricket and grasshopper spermatocytes were determined as a function of the distance from the pole toward which the movement was directed. In the artificially produced univalents of cricket cells, correlation coefficients for ra...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329921
更新日期:1978-11-22 00:00:00
abstract::The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327377
更新日期:1978-10-20 00:00:00
abstract::By using the optical properties of birefringence of DNA, the arrangement of these molecules has been studied in Dinoflagellate chromosomes and Dipteran polytene chromosomes. These latter are used, here, as a reference material. These observations have been made under a polarizing microscope on intact and stretched chr...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330371
更新日期:1978-08-21 00:00:00
abstract::The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293176
更新日期:1978-06-28 00:00:00
abstract::A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328531
更新日期:1978-05-16 00:00:00
abstract::Metaphase chromosomes prepared from colcemid-treated mouse L929 cells by non-ionic detergent lysis exhibit distinct heterochromatic centromere regions and associated kinetochores when viewed by whole mount electron microscopy. Deoxyribonuclease I treatment of these chromosomes results in the preferential digestion of ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330554
更新日期:1978-04-25 00:00:00
abstract::Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff d...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329465
更新日期:1978-01-16 00:00:00
abstract::When A-banded human chromosomes mounted in water are transferred to an organic mounting medium, the chromosomes show uniform bright quinacrine fluorescence. This change is reversible. It is inferred that quinacrine is bound uniformly along the chromosomes, and that Q-banding is a consequence of a non-uniform distribut...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294941
更新日期:1977-12-06 00:00:00
abstract::Human satellite DNAs I, II and IV were transcribed to yield radioactive complementary RNAs (cRNAs). These cRNAs were hybridised to metaphase chromosomes of man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus). The results of this in situ hybridisation were analysed quantitativel...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327453
更新日期:1977-09-27 00:00:00
abstract::Chromosome fibers isolated from lymphocyte nuclei and prepared for electron microscopy by techniques designed to preserve their native structure have a distinctly knobby appearance, suggesting that DNA and protein are not distributed evenly along the fiber axis. Individual knobs (superbeads) are arranged in tandem and...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327030
更新日期:1977-07-18 00:00:00
abstract::Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00286046
更新日期:1977-07-08 00:00:00
abstract::The large heterochromatic chromosome of Drosophila nasutoides reveals distinctive C- and Q-bands. The regions which are negative in C-banding appear positive in Q-banding. The isochromosomic nature of this chromosome and the locality of the satellite DNAs in this chromosome are discussed with respect to these banding ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292680
更新日期:1977-04-27 00:00:00
abstract::Antitubulin antibody was used as an immunofluorescent and immunoelectron microscopic probe to localize tubulin in components of the mitotic apparatus of rat kangaroo (strain PtK1) cells in vitro. In addition to the detection of tubulin in the spindle microtubules and centrioles, other structures were found to display ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329772
更新日期:1977-04-19 00:00:00
abstract::Experiments were designed to test whether nonrandom segregation of sister chromatids at mitosis has a role in the production of cell diversity during embryogenesis, Segregation was examined in vivo in retinal cells from embryonic chicks. Chromatids were labelled with bromouracil and stained by the fluorescence plus Gi...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00288461
更新日期:1977-03-16 00:00:00
abstract::Triplet conjugation occurs frequently when three different mating types of T. thermophila are mixed under appropriate conditions. Genetic studies show that fertilization is usually tripolar, with each conjugant contributing a pronucleus to one mate and receiving a pronucleus from the other. Triplets are less common an...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330410
更新日期:1977-03-07 00:00:00
abstract::By taking advantage of the fact that fluorescent light (FL) induces strand breaks only in bromodeoxyuridine(BrdUy-substituted DNA, and that those breaks eventually lead to the formation of sister chromatid exchanges (SCEs), the response of SCEs to FL was studied carefully in Chinese hamster chromosomes in which, out o...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292776
更新日期:1977-02-03 00:00:00
abstract::Membrane-free folded chromosomes were purified from log-phase cultures of Escherichia coli and prepared for electron microscopy by aqueous (Kleinschmidt and Zahn) spreading. The appearance of the chromosomes depended on the salt concentrations in spreading. At certain salt concentration, the chromosomes resembled rose...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328479
更新日期:1976-12-16 00:00:00
abstract::DNA reassociation kinetics were studied, by means of the hydroxyapatite chromatography method, for four species of Amphibians with different nuclear DNA content: Xenopus laevis (3 pg DNA per haploid genome) and Bufo bufo (7 pg) of the Anura subclass and Triturus cristatus (23 pg) and Necturus maculosus (52 pg) of the ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327706
更新日期:1976-12-06 00:00:00
abstract::Evidence is presented that chromosome ends are attached to the nuclear envelope prior to the formation of axial cores during early meiotic prophase in the grasshopper Brachystola magna. The attachment sites of distal and proximal chromosome ends are clustered in a small region of the inner nuclear envelope resulting i...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292844
更新日期:1976-11-29 00:00:00
abstract::Replication times for all important chromosome bands, of both types R and Q (277 structures) are analysed. The R-bands form a group of structures whose DNA replicates during the early S-phase, while the DNA situated in the Q-bands replicates during the late S-phase. There may not exist overlapping between replication ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293440
更新日期:1976-10-12 00:00:00
abstract::The DNA coding for 28 S and 18 S ribosomal RNA, including the spacer regions, has been isolated from calf (Bos taurus) thymus gland. The method used included shearing of the total DNA to a highly homogeneous size population, selective heat denaturation and S1 nuclease treatment to remove single stranded DNA. Repeated ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293439
更新日期:1976-10-12 00:00:00
abstract::Male hybrids of the cross D. azteca X D. athabasca are larger (hybrid giant males) than their parents, whereas hybrid females are of the same size as the parental species. Microspectrophotometric measurements have shown that the larval polytene salivary gland chromosomes of hybrid giant males undergo one more endorepl...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00295210
更新日期:1976-09-10 00:00:00
abstract::BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolut...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292912
更新日期:1976-08-17 00:00:00
abstract::The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromoso...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293114
更新日期:1976-06-30 00:00:00
abstract::Mitomycin C-induced sister chromatic exchange and quadriradial formation were studied in chromosomes from Muntiacus muntjac fibroblasts and A9, a transformed mouse cell line. We present the first direct cytological evidence for the induction of true somatic recombination involving homologous chromosomes. Analysis of t...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293724
更新日期:1976-06-30 00:00:00
abstract::The salivary chromosomes of the l(3)tl mutant of D. melanogaster are considerably thicker and shorter than those of normal larvae. In most nuclei, chromosome shortening is associated with morphological changes of two types. a) The bands lose their distinctive pattern and become loose. The chromosome appears as a granu...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01798344
更新日期:1976-04-21 00:00:00
abstract::The method of ultrathin sections of unsquashed salivary gland polytene chromosomes of Ch. thummi was applied to their ultrastrual mapping. There was a good agreement between electron micrographs and Hägele's light microscopic map (1970) with respect to the pattern and number of bands. 94% of bands were identified in l...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00288332
更新日期:1976-03-31 00:00:00
abstract::The genus Caledia acontains two species. C. species nova 1 is restricted to the Oriomo Plateau of S. W. Papua and has a complement of twelve telocentric chromosomes. The second species C. captiva has a much wider distribution pattern--from S.W. Papua in the North, down the entire Eastern seaboard of Australia to South...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293452
更新日期:1976-02-23 00:00:00
abstract::After conjugation in the ciliated protozoan, Oxytricha, polytene chromosomes are formed during the development of a macronucleus from a micronucleus. Here we report a microscopic study of these chromosomes and an analysis of their DNA. The polytene chromosomes of Oxytricha bear a strong morphological resemblance to th...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00331828
更新日期:1976-01-27 00:00:00
abstract::Quinacrine (Q-band) and centromeric heterochromatin (C-band) patterns of metaphase chromosomes of two subspecies of Mus musculus were compared. M.m. musculus (the laboratory mouse) and M.m. molossinus (a subspecies from Southeast Asia) had similar Q-band patterns along the length of the chromosomes, but differences we...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294081
更新日期:1975-12-29 00:00:00
abstract::In order to induce chromosomal rearrangements, males were exposed to x-rays and then mated to non-irradiated females. The number of each type of structural alteration was determined by examination of the polytene chromosomes of the F1 progeny. -- A comparison of the results with similar studies made on Drosophila reve...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329175
更新日期:1975-12-10 00:00:00
abstract::Macronuclear DNA was isolated from purified macronuclei of Paramecium aurelia and the size distribution was determined with regard to growth phase and method of extraction. DNA molecules as long as 105 microns and as short as 0.2 microns were observed. It was concluded that the method of extraction affected the observ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329171
更新日期:1975-12-10 00:00:00
abstract::In situ hybridization with 3H 18S and 28S ribosomal RNA from Xenopus laevis has been used to study the distribution of DNA sequences coding for these RNAs (the nucleolus organizing regions) in the genomes of six mammals. Several patterns of distribution have been found: 1) A single major site (rat kangaroo, Seba's fru...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329388
更新日期:1975-11-20 00:00:00
abstract::Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00364018
更新日期:1975-11-11 00:00:00
abstract::The interaction of Hoechst 33258 with DNA has been examined to help clarify the mechanisms of banding. 1. In agreement with previous studies Hoechst fluorescence is enhanced to a greater degree in AT-rich compared to GC-rich DNA. 2. Hoechst causes an increase in the DNA Tm which is greater at the higher AT content of ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00332113
更新日期:1975-10-14 00:00:00